The DENND1C Polyclonal Antibody (PAC052518) is a crucial tool for researchers studying DENND1C, a protein involved in intracellular membrane trafficking and vesicle formation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the DENND1C protein, it enables accurate detection and analysis in various cell types, making it an indispensable resource for research in cell biology and molecular biology.DENND1C, also known as DENN domain-containing protein 1C, is essential for regulating the transport of cellular components and maintaining proper cell function.
Its role in intracellular trafficking makes it a key target for investigating cellular processes and understanding diseases related to trafficking dysfunction. By studying DENND1C, researchers can gain valuable insights into conditions such as neurodegenerative disorders, metabolic diseases, and cancer, potentially leading to the development of targeted therapies and treatments.
Western Blot. Positive WB detected in: Rat liver tissue, Rat lung tissue. All lanes: DENND1C antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 88, 83 kDa. Observed band size: 88 kDa.
Immunohistochemistry of paraffin-embedded human lung tissue using PACO52518 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human lymph node tissue using PACO52518 at dilution of 1:100.
Background:
Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
Synonyms:
DENN domain-containing protein 1C (Connecdenn 3) (Protein FAM31C), DENND1C, FAM31C
UniProt Protein Function:
DENND1C: a guanine nucleotide exchange factor (GEF) associated with clathrin-associated vesicles. Appears to activate RAB35, RAB8A, and RAB13. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Exhibits low nucleotide-independent RAB35-binding activity. Interacts with clathrin heavy chain/CLTC and with AP2A2, but not with AP2B1. 2 isoforms of the human protein are produced by alternative splicing.Protein type: GEFs, Rab; GEFsChromosomal Location of Human Ortholog: 19p13.3Cellular Component: clathrin-coated vesicle; cytosolMolecular Function: Rab guanyl-nucleotide exchange factor activity
UniProt Protein Details:
NCBI Summary:
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1C, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]
