The DEFA5 Polyclonal Antibody (CAB18208) is a valuable tool for researchers studying the DEFA5 protein, which is involved in the innate immune response and has antimicrobial properties. This rabbit-derived antibody is highly specific for human samples and has been validated for use in Western blot applications, enabling detection and analysis of DEFA5 in various cell types.DEFA5, also known as human alpha defensin 5, plays a crucial role in the host defense mechanism by targeting and killing pathogens such as bacteria and viruses.
Its importance in the innate immune system makes it an interesting target for research in infectious diseases, inflammatory disorders, and immune-mediated conditions. By understanding the function and regulation of DEFA5, scientists can develop novel therapeutic strategies for combating infections and managing immune-related disorders.
Product Name:
DEFA5 Rabbit Polyclonal Antibody
SKU:
CAB18208
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-94 of human DEFA5 (NP_066290.1).
extracellular region, extracellular space, Golgi lumen, transport vesicle
Calculated MW:
10kDa
Observed MW:
12kDa
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum.
Purification Method:
Affinity purification
Gene ID:
1670
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HCT116 cells, using DEFA5 antibody (CAB18208) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
