The DDX58 Polyclonal Antibody (CAB0550) is a valuable tool for researchers studying the DDX58 protein, also known as RIG-I, which plays a crucial role in the innate immune response to viral infections. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.The DDX58 antibody binds to the DDX58 protein, allowing for detection and analysis in various cell types.
Its high reactivity and specificity make it ideal for studies in virology, immunology, and infectious diseases. Understanding the function and regulation of DDX58 is essential for developing novel antiviral therapies and improving our overall understanding of the immune response to viral pathogens.
Product Name:
RIG-I/DDX58 Rabbit Polyclonal Antibody
SKU:
CAB0550
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 825-925 of human Rig-I/DDX58 (NP_055129.2).
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Purification Method:
Affinity purification
Gene ID:
23586
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Rig-I/DDX58 Rabbit pAb (CAB0550) at 1:4000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
