The DDX5 Polyclonal Antibody (CAB5296) is a valuable tool for researchers studying the DDX5 protein, a member of the DEAD-box RNA helicase family known for its role in RNA metabolism and gene expression regulation. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications.The DDX5 Polyclonal Antibody specifically binds to the DDX5 protein, enabling researchers to detect and analyze its expression in various cell types. This makes it a valuable asset for studies in molecular biology, RNA biology, and cancer research, where DDX5 has been implicated in tumor progression and metastasis.
Understanding the function and regulation of DDX5 is vital for unraveling its involvement in various cellular processes and disease states. By using the DDX5 Polyclonal Antibody, researchers can further investigate the role of DDX5 in gene expression, RNA processing, and cancer biology, potentially leading to the development of novel therapeutic strategies targeting this important protein.
Product Name:
DDX5 Rabbit Polyclonal Antibody
SKU:
CAB5296
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-614 of human DDX5 (NP_004387.1).
This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1655
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DDX5 antibody (CAB5296) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
