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DDX42 Antibody (PACO37422)

SKU:
PACO37422
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • DDX42 Antibody PACO37422
  • DDX42 Antibody PACO37422
  • DDX42 Antibody PACO37422
$407
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Description

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DDX42 Antibody (PACO37422)

The DDX42 polyclonal antibody (PACO37422) is a valuable tool for researchers studying DDX42, a DEAD-box RNA helicase involved in RNA metabolism and RNA-binding protein regulation. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the DDX42 protein, this antibody allows for the detection and analysis of DDX42 in various cell types, making it an ideal choice for studies in RNA biology and cancer research.

DDX42 plays a crucial role in RNA processing and is implicated in various cellular processes, including mRNA splicing, ribosome biogenesis, and RNA transport. Dysregulation of DDX42 has been linked to cancer progression and other diseases, making it a promising target for therapeutic intervention. Understanding the function of DDX42 is essential for uncovering its potential role in disease pathogenesis and developing novel treatments targeted at this RNA helicase.

Antibody Name:DDX42 Antibody (PACO37422)
Antibody SKU:PACO37422
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:500
Species Reactivity:Human
Immunogen:Recombinant Human ATP-dependent RNA helicase DDX42 protein (519-819AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageImmunohistochemistry of paraffin-embedded human tonsil tissue using PACO37422 at dilution of 1:100.
Product ImageImmunofluorescence staining of Hela cells with PACO37422 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product ImageImmunohistochemistry of paraffin-embedded human lung tissue using PACO37422 at dilution of 1:100.
Background:ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
Synonyms:ATP-dependent RNA helicase DDX42 (EC 3.6.4.13) (DEAD box protein 42) (RNA helicase-like protein) (RHELP) (RNA helicase-related protein) (RNAHP) (SF3b DEAD box protein) (Splicing factor 3B-associated 125 kDa protein) (SF3b125), DDX42
UniProt Protein Function:DDX42: ATP-dependent RNA helicase. Binds to partially double- stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm. Belongs to the DEAD box helicase family. DDX42 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function:ATP-dependent RNA helicase activity; protein binding

Biological Process: protein localization; RNA secondary structure unwinding

NCBI Summary:This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q86XP3
NCBI GenInfo Identifier:74750541
NCBI Gene ID:11325
NCBI Accession:Q86XP3.1
UniProt Secondary Accession:Q86XP3,O75619, Q68G51, Q96BK1, Q96HR7, Q9Y3V8, A6NML1 A8KA43,
UniProt Related Accession:Q86XP3
Molecular Weight:90,068 Da
NCBI Full Name:ATP-dependent RNA helicase DDX42
NCBI Synonym Full Names:DEAD-box helicase 42
NCBI Official Symbol:DDX42  
NCBI Official Synonym Symbols:RHELP; RNAHP; SF3B8; DDX42P; SF3b125  
NCBI Protein Information:ATP-dependent RNA helicase DDX42
UniProt Protein Name:ATP-dependent RNA helicase DDX42
UniProt Synonym Protein Names:DEAD box protein 42; RNA helicase-like protein; RHELP; RNA helicase-related protein; RNAHP; SF3b DEAD box protein; Splicing factor 3B-associated 125 kDa protein; SF3b125
Protein Family:ATP-dependent RNA helicase
UniProt Gene Name:DDX42  
UniProt Entry Name:DDX42_HUMAN
ELISA Kits
Human DDX42 / ATP-dependent RNA helicase DDX42 ELISA Kit
Human ATP-dependent RNA helicase DDX42 (DDX42) ELISA Kit
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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