The DDX18 Polyclonal Antibody (PAC061213) is a valuable tool for researchers studying the DDX18 protein, a member of the DEAD-box RNA helicase family that plays a critical role in RNA metabolism. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications.The DDX18 antibody binds to the DDX18 protein, enabling researchers to detect and analyze its expression in various cell types. This makes it an essential tool for studies in molecular biology, RNA processing, and gene regulation.
Understanding the function of DDX18 is crucial for advancing our knowledge of cellular processes and developing targeted therapies for diseases such as cancer and genetic disorders.With its high specificity and sensitivity, the DDX18 Polyclonal Antibody is a reliable tool for researchers looking to explore the role of DDX18 in health and disease. Its versatility and reliability make it a valuable asset in the fields of biochemistry, cell biology, and molecular genetics.
Antibody Name:
DDX18 Antibody (PACO61213)
Antibody SKU:
PACO61213
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human ATP-dependent RNA helicase DDX18 protein (32-110AA)
Immunofluorescence staining of Hela cells with PACO61213 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Probable RNA-dependent helicase.
Synonyms:
ATP-dependent RNA helicase DDX18 (EC 3.6.4.13) (DEAD box protein 18) (Myc-regulated DEAD box protein) (MrDb), DDX18, cPERP-D
UniProt Protein Function:
DDX18: Probable RNA-dependent helicase. Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.Protein type: EC 3.6.4.13; Nucleolus; RNA-binding; HelicaseChromosomal Location of Human Ortholog: 2q14.1Cellular Component: membrane; nucleolusMolecular Function: protein binding; ATP binding; ATP-dependent RNA helicase activityBiological Process: metabolic process
UniProt Protein Details:
NCBI Summary:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008]
