The DDX11 Polyclonal Antibody (CAB7666) is a vital tool for research involving DDX11, a DNA helicase enzyme that plays a crucial role in DNA replication and repair processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the DDX11 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it perfect for studies in genetics, cancer research, and molecular biology.DDX11, also known as ChlR1 (Chl1-like protein 1), is essential for maintaining genomic stability and preventing the accumulation of genetic mutations that can lead to diseases like cancer.
By studying the function of DDX11, researchers can gain insights into the mechanisms underlying DNA replication and repair, as well as potential therapeutic targets for cancer treatment. Understanding the role of DDX11 in DNA metabolism is crucial for advancing our knowledge of genetic diseases and developing targeted therapies for various conditions.
Product Name:
DDX11 Rabbit Polyclonal Antibody
SKU:
CAB7666
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX11 (NP_689651.1).
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. This gene is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
1663
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DDX11 antibody (CAB7666) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
