The DDX1 Polyclonal Antibody (CAB6575) is a powerful tool for researchers interested in studying DDX1, a protein involved in RNA metabolism and RNA virus replication. This antibody, generated in rabbits, is highly specific to DDX1 and is suitable for use in various applications such as Western blot and immunohistochemistry.DDX1 is a member of the DEAD-box RNA helicase family and is known to play a role in RNA processing and RNA virus replication. By targeting DDX1 with this antibody, researchers can gain valuable insights into the function and regulation of this important protein in cellular processes such as RNA splicing, translation, and RNA virus replication.
The DDX1 Polyclonal Antibody is validated for use in human samples and has the potential to advance research in virology, RNA biology, and cancer biology. Its ability to specifically detect DDX1 protein in various cell types makes it a versatile tool for studying the molecular mechanisms underlying RNA metabolism and virus replication. Researchers interested in understanding the role of DDX1 in these processes will find this antibody essential for their studies.
Product Name:
DDX1 Rabbit Polyclonal Antibody
SKU:
CAB6575
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1).
WB,1:1000 - 1:5000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsIHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:
DBP-RB; UKVH5d; DDX1
Positive Sample:
293F
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Cytoplasmic granule, Nucleus.
Calculated MW:
82kDa
Observed MW:
83kDa
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that acts as an ATP-dependent RNA helicase that has been found to promote coronaviruses replication.
Purification Method:
Affinity purification
Gene ID:
1653
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F cells, using DDX1 Rabbit pAb (CAB6575) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
