The Ddit4 Polyclonal Antibody (CAB14135) is a valuable tool for researchers studying the DDIT4 (DNA-damage-inducible transcript 4) protein, which is involved in cellular stress responses and the regulation of cell growth and survival. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in immunohistochemistry and Western blotting experiments.DDIT4 is a key player in the cellular stress response pathway, acting as a negative regulator of the mammalian target of rapamycin (mTOR) complex 1.
By inhibiting mTOR signaling, DDIT4 helps cells adapt to various stress stimuli, such as nutrient deprivation, hypoxia, and DNA damage. Dysregulation of DDIT4 has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes.With its ability to specifically detect DDIT4 protein levels in various cell types and tissue samples, the Ddit4 Polyclonal Antibody (CAB14135) is a valuable tool for researchers looking to further explore the functions and therapeutic potential of DDIT4 in different disease contexts.
Product Name:
DDIT4 Rabbit Polyclonal Antibody
SKU:
CAB14135
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.
Purification Method:
Affinity purification
Gene ID:
54541
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DDIT4 antibody (CAB14135) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
