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Anti-DDIT3 / CHOP Antibody (CAB6504)

SKU:
CAB6504
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-DDIT3 / CHOP Antibody CAB6504
  • Cell Death Antibodies 2 Anti-DDIT3 / CHOP Antibody CAB6504
  • Cell Death Antibodies 2 Anti-DDIT3 / CHOP Antibody CAB6504
  • Cell Death Antibodies 2 Anti-DDIT3 / CHOP Antibody CAB6504
  • Cell Death Antibodies 2 Anti-DDIT3 / CHOP Antibody CAB6504
€139 - €419
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Description

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Anti-DDIT3 / CHOP Antibody (CAB6504)

The DDIT3 Polyclonal Antibody (CAB6504) is a valuable tool for researchers studying the DNA damage-inducible transcript 3 (DDIT3) protein. DDIT3, also known as CHOP (C/EBP homologous protein), is a transcription factor involved in the cellular response to stress, including endoplasmic reticulum (ER) stress and DNA damage. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples. It is validated for use in multiple applications, including Western blot and immunofluorescence, enabling the detection and analysis of DDIT3 in various cell types. The DDIT3 protein is a key player in regulating cell fate decisions in response to stress, including promoting apoptosis in response to severe or prolonged stress.

Dysregulation of DDIT3 has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic diseases. Therefore, understanding the function and regulation of DDIT3 is crucial for developing targeted therapies for these conditions.Overall, the DDIT3 Polyclonal Antibody (CAB6504) provides researchers with a reliable tool for studying the role of DDIT3 in cellular stress responses and disease pathogenesis.

Antibody Name:Anti-DDIT3 / CHOP Antibody
Antibody SKU:CAB6504
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human DDIT3 / CHOP
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:C2C12, C6
Immunogen:Recombinant protein of human DDIT3 / CHOP
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1649
Uniprot:P35638
Cellular Location:Cytoplasm, Nucleus
Calculated MW:19kDa/21kDa
Observed MW:30KDa
Synonyms:DDIT3, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153, C/EBPzeta, DDIT3 / CHOP
Background:This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
UniProt Protein Function:CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.
UniProt Protein Details:

Protein type:Autophagy; Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:cAMP response element binding protein binding; DNA binding; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity; transcription factor binding

Biological Process: cell cycle arrest; cell redox homeostasis; inhibition of CREB transcription factor; mRNA transcription from RNA polymerase II promoter; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of interleukin-8 production; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription in response to stress; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to unfolded protein

Disease: Myxoid Liposarcoma

NCBI Summary:This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
UniProt Code:P35638
NCBI GenInfo Identifier:544022
NCBI Gene ID:1649
NCBI Accession:P35638.1
UniProt Secondary Accession:P35638,F8VS99,
UniProt Related Accession:P35638
Molecular Weight:21,700 Da
NCBI Full Name:DNA damage-inducible transcript 3 protein
NCBI Synonym Full Names:DNA damage inducible transcript 3
NCBI Official Symbol:DDIT3  
NCBI Official Synonym Symbols:CHOP; CEBPZ; CHOP10; CHOP-10; GADD153  
NCBI Protein Information:DNA damage-inducible transcript 3 protein
UniProt Protein Name:DNA damage-inducible transcript 3 protein
UniProt Synonym Protein Names:C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153
Protein Family:DNA damage-inducible transcript 3 protein
UniProt Gene Name:DDIT3  
UniProt Entry Name:DDIT3_HUMAN
Anti-DDIT3 / CHOP Antibody (CAB6504)Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (CAB6504) at 1:1000 dilution.Both C2C12 cells and C6 cells were treated by tunicamycin (2 ug/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-DDIT3 / CHOP Antibody (CAB6504)Immunohistochemistry of paraffin-embedded human prostate cancer using DDIT3 / CHOP Rabbit pAb (CAB6504) at dilution of 1:200 (40x lens).
Anti-DDIT3 / CHOP Antibody (CAB6504)Immunohistochemistry of paraffin-embedded mouse kidney using DDIT3 / CHOP Rabbit pAb (CAB6504) at dilution of 1:200 (40x lens).
Anti-DDIT3 / CHOP Antibody (CAB6504)Immunohistochemistry of paraffin-embedded rat kidney using DDIT3 / CHOP Rabbit pAb (CAB6504) at dilution of 1:200 (40x lens).
Anti-DDIT3 / CHOP Antibody (CAB6504)Immunofluorescence analysis of C6 cells using DDIT3 / CHOP Rabbit pAb (CAB6504) at dilution of 1:100. Blue: DAPI for nuclear staining.

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