The DDB2 Polyclonal Antibody (CAB1848) is a valuable tool for researchers studying the DNA damage-binding protein 2 (DDB2), an essential component of the nucleotide excision repair pathway. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the DDB2 protein, researchers can accurately detect and analyze its expression levels in various cell types.
DDB2 is a key player in maintaining genomic stability by recognizing and initiating the repair of UV-induced DNA damage. Dysregulation of DDB2 has been implicated in various cancers, highlighting its importance in tumor suppression. By studying the function and regulation of DDB2, researchers can gain insights into the mechanisms of DNA repair and tumor development, potentially leading to the discovery of novel therapeutic targets for cancer treatment.
Product Name:
DDB2 Rabbit Polyclonal Antibody
SKU:
CAB1848
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1).
This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1643
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DDB2 antibody (CAB1848) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
