DDB1 Rabbit Monoclonal Antibody (CAB5022)
- SKU:
- CAB5022
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Synonyms:
- DDBA
- UV-DDB1
- XAP1
- XPCE
- XPE
- XPE-BF
Description
| Product Name: | DDB1 Rabbit Monoclonal Antibody |
| SKU: | CAB5022 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1041-1140 of human DDB1 (NP_001914.3). |
| Sequence: | TSLS ESWY NLLL DMQN RLNK VIKS VGKI EHSF WRSF HTER KTEP ATGF IDGD LIES FLDI SRPK MQEV VANL QYDD GSGM KREA TADD LIKV VEEL TRIH |
| Tested Applications: | WB IHC-P ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:500 - 1:1000 |
| Synonyms: | XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1; WHIKERS; DDB1 |
| Positive Sample: | HeLa,MCF7,NIH/3T3,C6,Mouse brain,Rat lung |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Nucleus |
| Calculated MW: | 127kDa |
| Observed MW: | 127kDa |
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
| Purification Method: | Affinity purification |
| Gene ID: | 1642 |
| Clone Number: | ARC1278 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of various cell lines, using DDB1 Rabbit mAb (CAB5022) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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