The DDB1 Polyclonal Antibody (CAB3827) is a valuable tool for researchers studying DDB1, a protein involved in DNA repair and genome stability. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DDB1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and cancer research.DDB1 is a crucial component of the E3 ubiquitin ligase complex involved in DNA damage recognition and repair. Dysregulation of DDB1 has been implicated in various diseases, including cancer and neurodegenerative disorders.
By understanding the function and regulation of DDB1, researchers can gain valuable insights into the mechanisms underlying these pathological conditions, potentially leading to the development of novel therapeutic approaches.With its high specificity and sensitivity, the DDB1 Polyclonal Antibody (CAB3827) is a powerful tool for investigating the role of DDB1 in cellular processes and disease pathogenesis. Its versatility and reliability make it a valuable asset for scientists conducting cutting-edge research in the fields of genetics, molecular biology, and oncology.
Product Name:
DDB1 Rabbit Polyclonal Antibody
SKU:
CAB3827
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1000-1140 of human DDB1 (NP_001914.3).
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
Purification Method:
Affinity purification
Gene ID:
1642
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DDB1 Rabbit pAb (CAB3827) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
