The DDB1 Polyclonal Antibody (CAB2896) is a specialized tool for studying the DDB1 protein, which plays a key role in the DNA damage response pathway. This antibody, produced in rabbits, has high specificity and sensitivity for detecting DDB1 in human samples, making it suitable for Western blot applications. By targeting the DDB1 protein, researchers can investigate its functions in DNA repair and maintenance, as well as its involvement in diseases such as cancer and genetic disorders.
This antibody is an essential tool for studying the molecular mechanisms underlying genome stability and the cellular response to DNA damage.
Product Name:
DDB1 Rabbit Polyclonal Antibody
SKU:
CAB2896
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 741-1140 of human DDB1 (NP_001914.3).
BT-474,SW620,293T,Mouse eye,Mouse testis,Mouse breast
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
127kDa
Observed MW:
127kDa
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
Purification Method:
Affinity purification
Gene ID:
1642
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DDB1 Rabbit pAb (CAB2896) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
