The DCX Polyclonal Antibody (CAB17469) is an essential tool for researchers studying the DCX protein, which is crucial for neurogenesis and neuronal migration in the developing brain. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in various applications including Western blotting and immunofluorescence.DCX, also known as Doublecortin, is a microtubule-associated protein that plays a key role in neural development, particularly in the formation of the cerebral cortex.
Mutations in the DCX gene have been linked to various neurological disorders such as lissencephaly and subcortical band heterotopia. By using the DCX Polyclonal Antibody, researchers can study the expression and localization of DCX in different cell types and tissues, providing valuable insights into brain development and neurological diseases.
Product Name:
DCX Rabbit Polyclonal Antibody
SKU:
CAB17469
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin (NP_835365.1).
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1641
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Doublecortin antibody (CAB17469) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): JurkatExposure time: 10s.
