The DCX Polyclonal Antibody (CAB14611) is a valuable tool for researchers studying DCX, a microtubule-associated protein that plays a crucial role in neuronal migration and differentiation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the DCX protein, this antibody allows for the detection and analysis of DCX in various cell types, making it ideal for research in neurobiology and neurodevelopmental disorders.
DCX is essential for the proper development of the nervous system, particularly in the migration of newly generated neurons to their final destination. Dysregulation of DCX has been linked to neurological disorders such as lissencephaly and epilepsy, making it a promising target for therapeutic interventions. By studying the function of DCX, researchers can gain insights into the mechanisms underlying these disorders and potentially develop novel treatments.
Product Name:
DCX Rabbit Polyclonal Antibody
SKU:
CAB14611
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin (NP_835365.1).
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1641
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Doublecortin antibody (CAB14611) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
