The Doublecortin Polyclonal Antibody is a valuable tool for researchers studying the protein doublecortin, which plays a critical role in neuronal migration and development in the brain. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to doublecortin, this antibody enables accurate detection and analysis of the protein in various cell types, making it ideal for neuroscience and developmental biology research.Doublecortin is essential for proper brain development, as mutations in the gene encoding this protein are associated with disorders such as lissencephaly and epilepsy.
Understanding the function and regulation of doublecortin is key to unraveling the mechanisms underlying these neurological conditions. The Doublecortin Polyclonal Antibody provides researchers with a reliable tool to investigate the role of doublecortin in brain development and function, ultimately leading to new insights into neurodevelopmental disorders.
Product Name:
Doublecortin Polyclonal Antibody
SKU:
CAB0149
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin (NP_835365.1).
This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1641
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse brain, using Doublecortin antibody (CAB0149).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
