The DCTN4 Polyclonal Antibody (PAC016157) is a valuable tool for research involving DCTN4, a subunit of the dynactin complex responsible for regulating cellular transport processes. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the DCTN4 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential reagent for studies in cell biology and molecular transport mechanisms.DCTN4 is known for its role in dynactin-mediated cargo transport along microtubules, impacting various cellular processes such as organelle positioning and intracellular trafficking.
Understanding the function of DCTN4 is crucial for unraveling its involvement in diseases like neurodegenerative disorders and cancer, where disruptions in cellular transport pathways contribute to pathogenesis. By studying DCTN4 with the PAC016157 antibody, researchers can gain insights into the mechanisms underlying these conditions and potentially identify targets for therapeutic interventions.
Antibody Name:
DCTN4 Antibody (PACO16157)
Antibody SKU:
PACO16157
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human DCTN4
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO16157(DCTN4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO16157(DCTN4 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein . Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end . The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1. Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin . In addition, Dynactin p62 is distributed in the nucleus at very high expression levels . Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton.
Synonyms:
dynactin 4 (p62)
UniProt Protein Function:
dynactin 4: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton. Belongs to the dynactin subunit 4 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxis; Motor; Microtubule-bindingChromosomal Location of Human Ortholog: 5q33.1Cellular Component: centrosome; cytoplasm; cytoplasmic dynein complex; cytosol; dynactin complex; nucleusMolecular Function: protein binding; protein N-terminus bindingBiological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; ER to Golgi vesicle-mediated transport; nuclear migration
