The DCTN4 Polyclonal Antibody (PAC016156) is a valuable tool for researchers studying the function of dynactin subunit 4 (DCTN4), a protein involved in intracellular transport and cargo binding. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it suitable for use in Western blot applications. By targeting DCTN4, researchers can accurately detect and analyze the protein in various cell types, providing essential insights into cellular processes related to organelle transport and cellular organization.
DCTN4 is a key component of the dynactin complex, essential for the regulation of cytoplasmic dynein motor activity and microtubule-based transport. Dysregulation of DCTN4 has been linked to various diseases, including neurodegenerative disorders and cancer, highlighting its importance in cellular function and disease pathology. Investigating the role of DCTN4 using this antibody can lead to a better understanding of its involvement in these conditions and potentially identify new therapeutic targets for intervention.
Antibody Name:
DCTN4 Antibody (PACO16156)
Antibody SKU:
PACO16156
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human DCTN4
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO16156(DCTN4 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO16156(DCTN4 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein . Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end . The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1. Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin . In addition, Dynactin p62 is distributed in the nucleus at very high expression levels . Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton.
Synonyms:
dynactin 4 (p62)
UniProt Protein Function:
dynactin 4: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton. Belongs to the dynactin subunit 4 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxis; Motor; Microtubule-bindingChromosomal Location of Human Ortholog: 5q33.1Cellular Component: centrosome; cytoplasm; cytoplasmic dynein complex; cytosol; dynactin complex; nucleusMolecular Function: protein binding; protein N-terminus bindingBiological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; ER to Golgi vesicle-mediated transport; nuclear migration
