The DCT Polyclonal Antibody (CAB16041) is a valuable tool for research involving DCT, a key enzyme involved in melanin biosynthesis. This antibody, raised in rabbits, is highly specific and reacts well with human samples, making it suitable for various applications such as Western blot and immunohistochemistry.DCT, also known as dopachrome tautomerase, plays a crucial role in the melanogenesis pathway by catalyzing the conversion of dopachrome to dihydroxyindole carboxylic acid (DHICA). This process is essential for the production of eumelanin, the dark pigment responsible for determining skin, hair, and eye color.
Research on DCT is particularly relevant in the fields of dermatology, pigmentation disorders, and cancer biology. Understanding the regulation and function of DCT can provide insights into melanin production, skin pigmentation, and potential therapeutic targets for conditions such as melanoma.By utilizing the DCT Polyclonal Antibody, researchers can accurately detect and analyze DCT expression in various cell types and tissues, advancing our understanding of melanin biosynthesis and its implications in health and disease.
Product Name:
DCT Rabbit Polyclonal Antibody
SKU:
CAB16041
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human DCT (NP_001913.2).
Melanosome membrane, Single-pass type I membrane protein
Calculated MW:
59kDa
Observed MW:
59kDa
Predicted to enable dopachrome isomerase activity. Involved in response to blue light. Located in intracellular membrane-bounded organelle and plasma membrane. Implicated in oculocutaneous albinism.
Purification Method:
Affinity purification
Gene ID:
1638
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DCT Rabbit pAb (CAB16041) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
