The DCLRE1B Polyclonal Antibody (CAB6808) is a valuable tool for researchers studying DNA cross-link repair enzyme 1B (DCLRE1B), a key protein involved in DNA repair processes. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to DCLRE1B, this antibody enables researchers to detect and analyze the protein in various cell types, making it a versatile tool for studies in genetics and cancer research.
DCLRE1B plays a crucial role in maintaining genomic stability by repairing DNA damage caused by cross-links between DNA strands. Mutations in DCLRE1B have been linked to increased susceptibility to cancer and other genetic disorders, highlighting the importance of understanding its function. By investigating the activity of DCLRE1B, researchers can gain insights into the mechanisms of DNA repair and potentially develop targeted therapies for genetic diseases and cancer.
Product Name:
DCLRE1B Rabbit Polyclonal Antibody
SKU:
CAB6808
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 323-532 of human DCLRE1B (NP_073747.1).
Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, telomere.
Calculated MW:
60kDa
Observed MW:
60kDa
DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).
Purification Method:
Affinity purification
Gene ID:
64858
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of THP-1 cells, using DCLRE1B antibody (CAB6808) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
