The DCLK1 Polyclonal Antibody (CAB9474) is a valuable tool for researchers studying DCLK1, a protein involved in tumor growth and progression. This antibody, produced in rabbits, is highly specific to human DCLK1 and has been validated for use in various applications, including Western blot and immunohistochemistry.DCLK1, also known as doublecortin-like kinase 1, is a potential therapeutic target in cancer due to its role in promoting tumor development and metastasis. By targeting DCLK1 with this antibody, researchers can better understand its function and potential as a biomarker or treatment target in various cancer types.
The antibody's ability to detect and analyze DCLK1 in different cell types makes it ideal for cancer research studies focused on tumor biology, metastasis, and targeted therapy development. By utilizing this antibody, researchers can gain valuable insights into the role of DCLK1 in cancer progression and potentially identify new strategies for treating cancer.
Product Name:
DCAMKL1/DCLK1 Rabbit Polyclonal Antibody
SKU:
CAB9474
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 590-740 of DCAMKL1/DCLK1 (NP_001317000.1).
This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.
Purification Method:
Affinity purification
Gene ID:
9201
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using DCAMKL1/DCLK1 antibody (CAB9474) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
