The DCHS1 Polyclonal Antibody (PAC063471) is a valuable tool for researchers studying DCHS1, a cell adhesion molecule involved in tissue development and morphogenesis. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DCHS1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for studies in developmental biology and cancer research.DCHS1 is known for its role in regulating cell polarity and tissue organization during embryonic development, making it a critical player in processes such as neural tube formation and organogenesis.
Its importance in tissue architecture and cell adhesion makes it a key target for investigating developmental disorders, cancer progression, and tissue regeneration. Understanding the function of DCHS1 is essential for uncovering its potential implications in these contexts and developing innovative therapies targeting this molecule.
IHC image of PACO63471 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of SH-SY5Y cells with PACO63471 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Calcium-dependent cell-adhesion protein. Mediates functions in neuroprogenitor cell proliferation and differentiation. In the heart, has a critical role for proper morphogenesis of the mitral valve, acting in the regulation of cell migration involved in valve formation.
