The DBI Polyclonal Antibody (CAB5370) is a specialized research tool for studying DBI, a protein involved in various cellular processes, including stress response and neurotransmission regulation. This antibody is produced in rabbits and has high specificity for human samples, making it ideal for use in Western blot applications to detect and analyze DBI expression in different cell types.DBI, also known as diazepam-binding inhibitor, is a multifunctional protein that interacts with various molecules, including benzodiazepines, to modulate their effects on neurotransmission and cell signaling. Its involvement in stress and anxiety regulation, as well as its potential role in neurological disorders, makes it an intriguing target for further research in neuroscience and pharmacology.
By using the DBI Polyclonal Antibody, researchers can gain valuable insights into the functions and mechanisms of DBI in normal physiological processes and pathological conditions. This antibody is a valuable tool for investigating the role of DBI in neurobiology, psychiatry, and drug development, offering new opportunities for advancing our understanding of the complex interactions within the nervous system.
Product Name:
DBI Rabbit Polyclonal Antibody
SKU:
CAB5370
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human DBI (NP_001171513.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EP; ACBP; ACBD1; CCK-RP; DBI
Positive Sample:
HeLa,HepG2,U-87MG
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum, Golgi apparatus.
Calculated MW:
10kDa
Observed MW:
12kDa
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
1622
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using DBI Rabbit pAb (CAB5370) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
