The DBI Polyclonal Antibody (CAB13293) is a highly specific research tool designed for the detection of DBI, a protein involved in various cellular processes. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is suitable for use in Western blot applications. By binding to the DBI protein, researchers can accurately detect and analyze this target in a variety of cell types, making it an excellent choice for studies in cell biology and neurobiology.DBI, also known as diazepam binding inhibitor, plays a crucial role in the regulation of GABAergic neurotransmission and has been implicated in processes such as anxiety, sleep, and memory.
Understanding the function of DBI is essential for unraveling its role in neurological disorders and potentially developing new therapeutic strategies. With its high specificity and sensitivity, the DBI Polyclonal Antibody is a valuable tool for investigating the intricate mechanisms involving DBI and its impact on brain function.
Product Name:
DBI Rabbit Polyclonal Antibody
SKU:
CAB13293
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human DBI (NP_001171513.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EP; ACBP; ACBD1; CCK-RP; DBI
Positive Sample:
HeLa,HepG2,U-87MG,Rat testis,Rat ovary
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum, Golgi apparatus.
Calculated MW:
10kDa
Observed MW:
11kDa
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Purification Method:
Affinity purification
Gene ID:
1622
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DBI Rabbit pAb (CAB13293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
