The DBI Monoclonal Antibody (CAB23242) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the CD300A protein, a cell surface molecule with important implications in immunology and cancer research. Developed using advanced technology, this monoclonal antibody offers high reactivity with human samples, providing researchers with accurate and reliable results in Western blot applications. By binding to the CD300A protein, this antibody enables precise detection and analysis in various cell types, offering a valuable tool for investigating immune homeostasis and inflammation regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DBI Monoclonal Antibody
SKU:
CAB23242
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC60030
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EP, ACBP, ACBD1, CCK-RP, DBI
Positive Sample:
HepG2, 293T, K-562
Cellular Localization:
Endoplasmic Reticulum, Golgi Apparatus.
Calculated MW:
10-12kDa
Observed MW:
12kDa
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
1622
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using DBI Rabbit mAb (CAB23242) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
