The DBF4B Polyclonal Antibody (PAC038330) is a valuable tool for researchers studying the DBF4B protein, a key player in cell cycle regulation and DNA replication processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to the DBF4B protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in molecular biology and cancer research.DBF4B is a crucial component of the pre-replication complex, playing a central role in DNA replication initiation and cell cycle progression.
Its involvement in these fundamental processes makes it a promising target for research into diseases like cancer, where dysregulation of cell division mechanisms can lead to uncontrolled growth and tumorigenesis. By gaining insights into the function of DBF4B, researchers can uncover new pathways for therapeutic intervention and potential targets for drug development in cancer treatment.
Western blot. All lanes: DBF4B antibody at 4µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 68, 48, 55, 19 kDa. Observed band size: 48 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO38330 at dilution of 1:100.
Background:
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
Synonyms:
Protein DBF4 homolog B (Activator of S phase kinase-like protein 1) (ASK-like protein 1) (Chiffon homolog B) (Dbf4-related factor 1), DBF4B, ASKL1 DRF1
