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Anti-DARS Antibody (CAB6574)

SKU:
CAB6574
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-DARS Antibody CAB6574
  • Metabolism Antibodies 2 Anti-DARS Antibody CAB6574
  • Metabolism Antibodies 2 Anti-DARS Antibody CAB6574
  • Metabolism Antibodies 2 Anti-DARS Antibody CAB6574
  • Metabolism Antibodies 2 Anti-DARS Antibody CAB6574
€139 - €419
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Description

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Antibody Name:Anti-DARS Antibody
Antibody SKU:CAB6574
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SW480, HepG2, Raji, Mouse liver, Mouse brain, Rat liver, 293T, U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KIIS AASE GGAN VFTV SYFK NNAY LAQS PQLY KQMC ICAD FEKV FSIG PVFR AEDS NTHR HLTE FVGL DIEM AFNY HYHE VMEE IADT MVQI FKGL QERF QTEI QTVN KQFP CEPF KFLE PTLR LEYC EALA MLRE AGVE MGDE DDLS TPNE KLLG HLVK EKYD TDFY ILDK YPLA VRPF YTMP DPRN PKQS NSYD MFMR GEEI LSGA QRIH DPQL LTER ALHH GIDL EKIK AYID SFRF GAPP HAGG GIGL ERVT MLFL GLHN VRQT SMFP RDPK RLTP
Gene ID:1615
Uniprot:P14868
Cellular Location:Cytoplasm
Calculated MW:45kDa/57kDa
Observed MW:57kDa
Synonyms:DARS, HBSL, aspRS
Background:This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:Translation; EC 6.1.1.12; Ligase

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity

Biological Process: tRNA aminoacylation for protein translation; translation; protein complex assembly; gene expression; aspartyl-tRNA aminoacylation

Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity

NCBI Summary:This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Code:P14868
NCBI GenInfo Identifier:20178330
NCBI Gene ID:1615
NCBI Accession:P14868.2
UniProt Secondary Accession:P14868,Q2TNI3, Q32Q69, Q53HV4, Q53YC5, Q68CR9, Q9BW52 A8K3J2, D3DP77,
UniProt Related Accession:P14868
Molecular Weight:501
NCBI Full Name:Aspartate--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:aspartyl-tRNA synthetase
NCBI Official Symbol:DARS  
NCBI Official Synonym Symbols:HBSL; aspRS  
NCBI Protein Information:aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein
UniProt Protein Name:Aspartate--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein
UniProt Gene Name:DARS  
UniProt Entry Name:SYDC_HUMAN
Anti-DARS Antibody (CAB6574)Western blot analysis of extracts of various cell lines, using DARS Antibody (CAB6574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-DARS Antibody (CAB6574)Immunohistochemistry of paraffin-embedded rat brain using DARS antibody (CAB6574) at dilution of 1:100 (40x lens).
Anti-DARS Antibody (CAB6574)Immunohistochemistry of paraffin-embedded mouse spinal cord using DARS antibody (CAB6574) at dilution of 1:100 (40x lens).
Anti-DARS Antibody (CAB6574)Immunohistochemistry of paraffin-embedded mouse brain using DARS antibody (CAB6574) at dilution of 1:100 (40x lens).
Anti-DARS Antibody (CAB6574)Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DARS antibody (CAB6574). Western blot was performed from the immunoprecipitate using DARS antibody (CAB6574) at a dilition of 1:1000.

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