The DARS Polyclonal Antibody (CAB6574) is a valuable tool for researchers studying DARS, a key enzyme involved in the synthesis of aspartyl-tRNA in aminoacyl-tRNA synthetase (ARS), which is essential for protein synthesis. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.DARS plays a crucial role in protein synthesis, making it a target of interest in studies focusing on translation regulation, amino acid metabolism, and genetic disorders associated with ARS dysfunction.
This antibody specifically binds to the DARS protein, allowing for accurate detection and analysis in a variety of cell types.With its potential applications in molecular biology, biochemistry, and genetic research, the DARS Polyclonal Antibody is an indispensable tool for exploring the functions and mechanisms of DARS in cellular processes. Its versatility and specificity make it an ideal choice for scientists studying protein synthesis and its implications in human health and disease.
Product Name:
DARS Rabbit Polyclonal Antibody
SKU:
CAB6574
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).
This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1615
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DARS Rabbit pAb (CAB6574) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
