The DAP3 Polyclonal Antibody (CAB7003) is a valuable tool for researchers studying DAP3, a key protein involved in apoptosis regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the DAP3 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential tool for studies in cell biology and cancer research.DAP3, also known as death-associated protein 3, is essential for the initiation of apoptosis, a critical process in maintaining cellular homeostasis and eliminating damaged cells.
Dysregulation of apoptosis is implicated in various diseases, including cancer, making DAP3 a promising target for therapeutic interventions. By understanding the role of DAP3 in apoptosis, researchers can develop novel strategies for treating diseases characterized by aberrant cell death processes.
Product Name:
DAP3 Rabbit Polyclonal Antibody
SKU:
CAB7003
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 159-398 of human DAP3 (NP_387506.1).
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.
Purification Method:
Affinity purification
Gene ID:
7818
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DAP3 Antibody (CAB7003) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
