The DAG1 Polyclonal Antibody (CAB21372) is a valuable tool for researchers studying the DAG1 protein, a key player in cell adhesion and signaling processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to DAG1, researchers can effectively detect and analyze this protein in various cell types, making it ideal for studies in developmental biology, neurology, and muscle diseases.
DAG1, also known as dystroglycan, is a critical component of the dystrophin-glycoprotein complex and is essential for maintaining muscle integrity and function. Mutations in DAG1 have been linked to muscular dystrophies and congenital muscular dystrophy, highlighting its importance in muscle biology. By understanding the role of DAG1 in cell adhesion and signaling pathways, researchers can uncover new insights into disease mechanisms and potential therapeutic strategies.
Product Name:
DAG1 Polyclonal Antibody
SKU:
CAB21372
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4).
This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.
Purification Method:
Affinity purification
Gene ID:
1605
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from SK-BR-3 cells, using DAG1 Rabbit pAb (CAB21372) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
