The DA (Dopamine) ELISA Kit is specifically designed for the precise measurement of dopamine levels in various biological samples, including serum, plasma, and tissue homogenates. This kit boasts exceptional sensitivity and specificity, guaranteeing consistent and accurate results for research purposes.Dopamine is a key neurotransmitter that plays vital roles in regulating mood, emotions, and cognitive functions. Imbalances in dopamine levels have been implicated in various neurological and psychiatric disorders, such as Parkinson's disease, schizophrenia, and addiction.
Therefore, this ELISA kit is essential for studying the role of dopamine in these conditions and exploring potential therapeutic interventions.Overall, the DA (Dopamine) ELISA Kit is a valuable tool for scientists and researchers interested in investigating the physiological and pathological roles of dopamine in the human body. Its reliability and precision make it an indispensable asset in the field of neuroscience and pharmacology.
Product Name:
DA (Dopamine) ELISA Kit (UNES00029)
SKU:
UNES00029
Target:
DA (Dopamine) (UNES00029)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DA. During the reaction, DA in the sample or standard competes with a fixed amount of DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
95-109
91-103
Average (%)
99
103
98
1:4
Range (%)
91-103
89-105
97-114
Average (%)
96
96
105
1:8
Range (%)
91-103
92-104
99-113
Average (%)
96
99
105
1:16
Range (%)
86-98
91-105
93-108
Average (%)
92
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.2
168.7
786.2
94.2
159.9
725.0
Standard deviation
6.1
7.4
29.1
5.3
9.0
37.0
C V (%)
6.55
4.39
3.7
5.63
5.63
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DA in samples. No significant cross-reactivity or interference between DA and analogues was observed.
