The D2HGDH Polyclonal Antibody (CAB16213) is a key tool for researchers studying D-2-hydroxyglutarate dehydrogenase (D2HGDH), an enzyme involved in the metabolism of D-2-hydroxyglutarate, a metabolite that has been implicated in various diseases including cancer and neurological disorders. This antibody, produced in rabbits, is highly specific to human D2HGDH and has been validated for use in Western blot applications. It binds specifically to the D2HGDH protein, enabling accurate detection and analysis in a variety of cell types, making it ideal for research in metabolic disorders, cancer biology, and neurobiology.
D2HGDH is a key enzyme in the metabolism of D-2-hydroxyglutarate, and dysregulation of this pathway has been associated with tumorigenesis and neurological disorders. Understanding the function and regulation of D2HGDH is crucial for developing targeted therapies for these diseases. The D2HGDH Polyclonal Antibody (CAB16213) is a valuable tool for researchers investigating the role of D2HGDH in health and disease.
Product Name:
D2HGDH Rabbit Polyclonal Antibody
SKU:
CAB16213
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 322-521 of human D2HGDH (NP_689996.4).
This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features.
Purification Method:
Affinity purification
Gene ID:
728294
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using D2HGDH Rabbit pAb (CAB16213) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
