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Cytokeratin 8 Polyclonal Antibody (CAB24434)

SKU:
CAB24434
€259
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Description

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Product Name:Cytokeratin 8 Polyclonal Antibody
SKU:CAB24434
Size:100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 380-480 of human Cytokeratin 8 (NP_002264.1).
Sequence:VKLA LDIE IATY RKLL EGEE SRLE SGMQ NMSI HTKT TSGY AGGL SSAY GGLT SPGL SYSL GSSF GSGA GSSS FSRT SSSR AVVV KKIE TRDG KLVS ESSD V
Tested Applications:WB
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; 8
Positive Sample:HeLa,Mouse large intestine
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, Nucleus matrix, nucleoplasm.
Calculated MW:54kDa
Observed MW:62kDa

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:3856
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Mouse large intestine, using Cytokeratin 8 Rabbit pAb (CAB24434) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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