The Cytochrome P450 3A4 Polyclonal Antibody (CAB2544) is a valuable tool for researchers studying the Cytochrome P450 3A4 enzyme, a key player in drug metabolism and detoxification in the liver. This antibody, developed through rabbit immunization, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the Cytochrome P450 3A4 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in pharmacology and toxicology research.
Cytochrome P450 3A4 is a major enzyme involved in the metabolism of a wide range of pharmaceutical drugs, environmental toxins, and endogenous compounds. Its role in drug interactions, drug efficacy, and drug toxicity underscores the importance of studying its expression and activity in different biological contexts. The use of this antibody allows researchers to better understand the function of Cytochrome P450 3A4 and its implications for drug development, personalized medicine, and drug response variability.
Product Name:
CYP3A4 Rabbit Polyclonal Antibody
SKU:
CAB2544
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
1576
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CYP3A4 Rabbit pAb (CAB2544) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
