Anti-CYP2E1 Antibody (CAB2160)

Antibody Name:	Anti-CYP2E1 Antibody
Antibody SKU:	CAB2160
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVIK NVAE VKEY VSER VKEH HQSL DPNC PRDL TDCL LVEM EKEK HSAE RLYT MDGI TVTV ADLF FAGT ETTS TTLR YGLL ILMK YPEI EEKL HEEI DRVI GPSR IPAI KDRQ EMPY MDAV VHEI QRFI TLVP SNLP HEAT RDTI FRGY LIPK GTVV VPTL DSVL YDNQ EFPD PEKF KPEH FLNE NGKF KYSD YFKP FSTG KRVC AGEG LARM ELFL LLCA ILQH FNLK PLVD PKDI DLSP IHIG FGCI PPRY KLCV IPRS
Gene ID:	1571
Uniprot:	P05181
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:	56kDa
Observed MW:	55kDa

Synonyms:

CYP2E1, CPE1, CYP2E, P450-J, P450C2E

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

UniProt Protein Function:	CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.13.n7; Lipid Metabolism - arachidonic acid; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Mitochondrial Chromosomal Location of Human Ortholog: 10q26.3 Cellular Component: endoplasmic reticulum membrane Molecular Function:arachidonic acid epoxygenase activity; enzyme binding; heme binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; oxygen binding; steroid hydroxylase activity Biological Process: benzene metabolic process; carbon tetrachloride metabolic process; drug metabolic process; epoxygenase P450 pathway; halogenated hydrocarbon metabolic process; heterocycle metabolic process; monoterpenoid metabolic process; steroid metabolic process; xenobiotic metabolic process
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]
UniProt Code:	P05181
NCBI GenInfo Identifier:	117250
NCBI Gene ID:	1571
NCBI Accession:	P05181.1
UniProt Secondary Accession:	P05181,Q5VZD5, Q6NWT9, Q9UK47,
UniProt Related Accession:	P05181
Molecular Weight:	56,849 Da
NCBI Full Name:	Cytochrome P450 2E1
NCBI Synonym Full Names:	cytochrome P450 family 2 subfamily E member 1
NCBI Official Symbol:	CYP2E1
NCBI Official Synonym Symbols:	CPE1; CYP2E; P450-J; P450C2E
NCBI Protein Information:	cytochrome P450 2E1
UniProt Protein Name:	Cytochrome P450 2E1
UniProt Synonym Protein Names:	4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-J
Protein Family:	Cytochrome
UniProt Gene Name:	CYP2E1
UniProt Entry Name:	CP2E1_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP2E1 antibody (CAB2160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody (CAB2160) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody (CAB2160) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using CYP2E1 antibody (CAB2160). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
CYP2E1 Antibody (PACO02542)	Human Cytochrome P450 2E1 (CYP2E1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)