null

Cytochrome P450 2D6 Colorimetric Cell-Based ELISA

SKU:
CBCAB01192
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Metabolism
Reactivity:
Human
Detection Method:
Colorimetric
€599
Frequently bought together:

Description

system_update_altDatasheetMSDS

Cytochrome P450 2D6 Colorimetric Cell-Based ELISA

The Cytochrome P450 2D6 Colorimetric Cell-Based ELISA Kit is a powerful tool for researchers looking to accurately measure the activity levels of the cytochrome P450 2D6 enzyme in cell samples. This kit offers high sensitivity and specificity, allowing for precise and reliable results that are essential for understanding drug metabolism, drug interactions, and personalized medicine.Cytochrome P450 2D6 is a key enzyme responsible for metabolizing a wide range of medications, including antidepressants, antipsychotics, and painkillers.

Variations in the activity of this enzyme can impact drug efficacy and safety, making it a critical biomarker for predicting individual responses to medications.With the Cytochrome P450 2D6 Colorimetric Cell-Based ELISA Kit, researchers can gain valuable insights into drug metabolism and personalized medicine, ultimately leading to improved patient outcomes and more effective treatment strategies.

Product Name:Cytochrome P450 2D6 Colorimetric Cell-Based ELISA
Product Code:CBCAB01192
ELISA Type:Cell-Based
Target:Cytochrome P450 2D6
Reactivity:Human
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The Cytochrome P450 2D6 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Cytochrome P450 2D6 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cytochrome P450 2D6 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on Cytochrome P450 2D6.

Qualitative determination of Cytochrome P450 2D6 concentration is achieved by an indirect ELISA format. In essence, Cytochrome P450 2D6 is captured by Cytochrome P450 2D6-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 1565, UniProt ID: P10635, OMIM: 124030, Unigene: Hs.648256
Gene Symbol:CYP2D6
Sub Type:None
UniProt Protein Function:CYP2D6: Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrion

Molecular Function:arachidonic acid epoxygenase activity; drug binding; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; steroid hydroxylase activity

Biological Process: alkaloid catabolic process; alkaloid metabolic process; arachidonic acid metabolic process; coumarin metabolic process; drug catabolic process; drug metabolic process; heterocycle metabolic process; isoquinoline alkaloid metabolic process; monoterpenoid metabolic process; negative regulation of binding; steroid metabolic process; xenobiotic metabolic process

Disease: Drug Metabolism, Poor, Cyp2d6-related

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:P10635
NCBI GenInfo Identifier:84028191
NCBI Gene ID:1565
NCBI Accession:P10635.2
UniProt Secondary Accession:P10635,Q16752, Q2XND6, Q2XND7, Q2XNE0, Q6B012, Q6NXU8
UniProt Related Accession:P10635
Molecular Weight:50,061 Da
NCBI Full Name:Cytochrome P450 2D6
NCBI Synonym Full Names:cytochrome P450 family 2 subfamily D member 6
NCBI Official Symbol:CYP2D6  
NCBI Official Synonym Symbols:CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1  
NCBI Protein Information:cytochrome P450 2D6
UniProt Protein Name:Cytochrome P450 2D6
UniProt Synonym Protein Names:CYPIID6; Cholesterol 25-hydroxylase
Protein Family:Cytochrome
UniProt Gene Name:CYP2D6  
UniProt Entry Name:CP2D6_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-Cytochrome P450 2D6 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

Related Products