The Cystatin B Polyclonal Antibody (CAB3815) is specifically designed for researchers studying Cystatin B, a protein involved in the regulation of protease activity and the inhibition of cysteine proteases. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications, making it a valuable tool for analyzing Cystatin B levels in various cell types.Cystatin B is known for its role in mediating the balance of protease activity, which is crucial for maintaining cellular functions and preventing abnormal tissue degradation.
Dysregulation of Cystatin B has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. By using the Cystatin B Polyclonal Antibody, researchers can gain insights into the expression and activity of Cystatin B, leading to a better understanding of its involvement in disease progression and potential therapeutic strategies.
Product Name:
CSTB Rabbit Polyclonal Antibody
SKU:
CAB3815
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTB (NP_000091.1).
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.
Purification Method:
Affinity purification
Gene ID:
1476
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CSTB Rabbit pAb (CAB3815) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
