CYPOR Polyclonal Antibody (CAB21845)
- SKU:
- CAB21845
Description
| Product Name: | CYPOR Polyclonal Antibody |
| SKU: | CAB21845 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3). |
| Sequence: | MINM GDSH VDTS STVS EAVA EEVS LFSM TDMI LFSL IVGL LTYW FLFR KKKE EVPE FTKI QTLT SSVR ESSF VEKM KKTG RNII VFYG SQTG TAEE FANR LSKD AHRY GMRG MSAD PEEY DLAD LSSL PEID NALV VFCM ATYG EGDP TDNA QDFY DWLQ ETDV DLSG VKFA VFGL GNKT YEHF NAMG KYVD KRLE QLGA QRIF ELGL GDDD GNLE EDFI TWRE QFWP AVCE HFGV EATG EESS IRQY EL |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | CPR; CYPOR; P450R |
| Positive Sample: | HeLa,Mouse liver |
| Conjugate: | Unconjugated |
| Cellular Localization: | Endoplasmic reticulum membrane, Peripheral membrane protein. |
| Calculated MW: | 77kDa |
| Observed MW: | 77kDa |
This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase.
| Purification Method: | Affinity purification |
| Gene ID: | 5447 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using CYPOR Rabbit pAb (CAB21845) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s. |
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