The CYPOR Polyclonal Antibody is a research tool designed for the study of cytochrome P450 oxidoreductase (CYPOR), a key enzyme involved in the metabolism of drugs and xenobiotics. This antibody, produced in rabbits, shows high specificity and reactivity towards human samples, making it an ideal choice for Western blot applications.CYPOR plays a crucial role in the cytochrome P450 system, which is responsible for the metabolism of a wide range of compounds, including drugs, toxins, and hormones. By targeting CYPOR, researchers can better understand the mechanisms involved in drug metabolism and potentially develop more effective treatments with fewer side effects.
This antibody enables the detection and analysis of CYPOR in various cell types, providing valuable insights into its function and activity. Its application in studies related to drug metabolism, toxicology, and pharmacology makes it a valuable tool for researchers in the fields of biochemistry, pharmacology, and drug development.
Product Name:
CYPOR Polyclonal Antibody
SKU:
CAB21845
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).
This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase.
Purification Method:
Affinity purification
Gene ID:
5447
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CYPOR Rabbit pAb (CAB21845) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
