CYP8B1 Antibody (PACO56776)
- SKU:
- PACO56776
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
CYP8B1 Antibody (PACO56776)
The CYP8B1 Polyclonal Antibody (PACO56776) is a crucial tool for researchers studying the cytochrome P450 enzyme family, with a specific focus on CYP8B1. This antibody, produced in rabbits, is highly specific and reacts strongly with human samples. Validated for use in Western blot applications, the antibody binds to the CYP8B1 protein, allowing for precise detection and analysis in various cell types.CYP8B1 is an important enzyme involved in bile acid synthesis and metabolism, playing a key role in lipid homeostasis and cholesterol regulation.
Dysregulation of CYP8B1 activity has been linked to various metabolic disorders, making it a promising target for research in liver diseases, diabetes, and cardiovascular conditions. Understanding the function of CYP8B1 is crucial for the development of potential therapeutics aimed at addressing these health issues.Overall, the CYP8B1 Polyclonal Antibody is an essential tool for researchers looking to investigate the role of CYP8B1 in metabolic pathways and explore its potential as a therapeutic target in various diseases.
| Antibody Name: | CYP8B1 Antibody (PACO56776) |
| Antibody SKU: | PACO56776 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase protein (303-440AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: CYP8B1 antibody at 6.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa. |
 | IHC image of PACO56776 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO56776 diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Involved in bile acid, synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid, and chenodeoxycholic acid, Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids. |
| Synonyms: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (EC 1.14.18.8) (7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase) (CYPVIIIB1) (Cytochrome P450 8B1) (Sterol 12-alpha-hydroxylase), CYP8B1, CYP12 |
| UniProt Protein Function: | CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.Protein type: Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, integral; EC 1.14.13.95; OxidoreductaseChromosomal Location of Human Ortholog: 3p22.1Cellular Component: endoplasmic reticulum membraneMolecular Function: 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity; oxygen binding; sterol 12-alpha-hydroxylase activityBiological Process: bile acid biosynthetic process; sterol metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UNU6 |
| NCBI GenInfo Identifier: | 308153428 |
| NCBI Gene ID: | 1582 |
| NCBI Accession: | Q9UNU6.2 |
| UniProt Secondary Accession: | Q9UNU6,O75958, Q6NWT2, Q6NWT3, B2RCY3 |
| UniProt Related Accession: | Q9UNU6 |
| Molecular Weight: | 58,068 Da |
| NCBI Full Name: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| NCBI Synonym Full Names: | cytochrome P450 family 8 subfamily B member 1 |
| NCBI Official Symbol: | CYP8B1Â Â |
| NCBI Official Synonym Symbols: | CP8B; CYP12Â Â |
| NCBI Protein Information: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| UniProt Protein Name: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| UniProt Synonym Protein Names: | 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase |
| Protein Family: | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| UniProt Gene Name: | CYP8B1Â Â |
| UniProt Entry Name: | CP8B1_HUMAN |
| Antibodies |
| CYP8B1 Antibody (PACO00673) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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