The CYP7B1 Polyclonal Antibody (CAB17872) is a valuable tool for researchers studying the CYP7B1 enzyme, which plays a key role in bile acid synthesis and cholesterol metabolism. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CYP7B1 protein, this antibody allows for the detection and analysis of CYP7B1 expression in various cell types, making it an essential tool for studies in lipid metabolism, liver function, and related diseases.CYP7B1 is a crucial enzyme in the conversion of cholesterol to bile acids, a process essential for lipid digestion and overall metabolic health.
Dysregulation of CYP7B1 activity has been linked to disorders such as hypercholesterolemia, atherosclerosis, and liver dysfunction. By investigating the function and regulation of CYP7B1, researchers can gain insights into these disease processes and potentially identify new therapeutic targets for intervention. The CYP7B1 Polyclonal Antibody is a reliable resource for furthering our understanding of this important enzyme and its role in human health.
Product Name:
CYP7B1 Rabbit Polyclonal Antibody
SKU:
CAB17872
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CYP7B1 (NP_004811.1).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder.
Purification Method:
Affinity purification
Gene ID:
9420
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of A-431, using CYP7B1 antibody (CAB17872) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
