The Anti-CYP7A1 Antibody (CAB10615) is a polyclonal antibody developed for research purposes involving the cytochrome P450 7A1 enzyme. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CYP7A1 enzyme, this antibody allows for the detection and analysis of CYP7A1 in various cell types, making it particularly useful for studies in the fields of metabolism, lipid homeostasis, and liver function.CYP7A1, also known as cholesterol 7 alpha hydroxylase, plays a crucial role in the metabolism of cholesterol by catalyzing the initial and rate-limiting step in the classical pathway of bile acid synthesis.
Dysregulation of CYP7A1 activity has been implicated in various metabolic disorders, including hypercholesterolemia and gallstone formation. Therefore, understanding the expression and function of CYP7A1 is essential for developing treatments for these conditions and advancing our knowledge of lipid metabolism.Overall, the Anti-CYP7A1 Antibody (CAB10615) serves as a valuable tool for researchers investigating the role of the CYP7A1 enzyme in health and disease, providing insights that may lead to the development of innovative therapies targeting dyslipidemia and related metabolic disorders.
Product Name:
CYP7A1 Rabbit Polyclonal Antibody
SKU:
CAB10615
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 390-504 of human CYP7A1 (NP_000771.2).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis.
Purification Method:
Affinity purification
Gene ID:
1581
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CYP7A1 antibody (CAB10615) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
