The CYP3A4 Polyclonal Antibody (CAB13484) is a valuable tool for researchers studying the cytochrome P450 enzyme CYP3A4, which is involved in the metabolism of a wide range of drugs and xenobiotics in the human body. This antibody, produced in rabbits, is highly specific to human CYP3A4 and is validated for use in Western blot and immunohistochemistry applications.CYP3A4 is one of the most important drug-metabolizing enzymes in the liver and intestines, playing a crucial role in determining the pharmacokinetics and bioavailability of many medications. Understanding the expression and activity of CYP3A4 is essential for predicting drug interactions, optimizing drug dosing, and minimizing the risk of adverse drug reactions.
By utilizing the CYP3A4 Polyclonal Antibody, researchers can accurately detect and analyze the expression of CYP3A4 in different tissues and cell types, shedding light on its role in drug metabolism and its potential as a target for drug development and personalized medicine approaches. This antibody is a valuable tool for studies in pharmacology, toxicology, and drug discovery, providing important insights into the complex interplay between drugs and the human body.
Product Name:
[KO Validated] CYP3A4 Rabbit Polyclonal Antibody
SKU:
CAB13484
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
1576
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat liver, using CYP3A4 antibody (CAB13484) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
