The CYP2A7 Polyclonal Antibody (PACO06399) is a valuable tool for researchers studying the CYP2A7 enzyme, a member of the cytochrome P450 family involved in drug metabolism and detoxification processes in the liver. Raised in rabbits, this antibody exhibits high specificity and sensitivity towards human samples, making it suitable for various research applications such as Western blot analysis.CYP2A7 plays a crucial role in the metabolism of a wide range of drugs, toxins, and endogenous compounds, making it a key player in drug interactions and toxicology studies. The ability to detect and analyze the expression of CYP2A7 in different cell types allows researchers to investigate its involvement in drug metabolism pathways and assess its potential impact on individual drug responses.
Furthermore, the dysregulation of CYP2A7 has been implicated in various disease states, including liver disorders, cancer, and cardiovascular diseases. By studying the expression and activity of CYP2A7 using this antibody, researchers can gain insights into the role of this enzyme in disease development and progression, ultimately paving the way for the development of targeted therapies and personalized medicine approaches.
Antibody Name:
CYP2A7 Antibody (PACO06399)
Antibody SKU:
PACO06399
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:40000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from the C-terminal region of human CYP2A7.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of Jurkat cells using CYP2A7 Polyclonal Antibody.
Western Blot analysis of 22RV1 cells using CYP2A7 Polyclonal Antibody.
CYP2A7: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.14.14.1; Endoplasmic reticulum; Secondary Metabolites Metabolism - caffeineChromosomal Location of Human Ortholog: 19q13.2Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasmMolecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen bindingBiological Process: coumarin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. This gene, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
