The Cytochrome P450 1A1 Polyclonal Antibody (CAB2159) is a valuable tool for researchers studying cytochrome P450 1A1, an enzyme involved in the metabolism of various substances including drugs, toxins, and carcinogens. This antibody, raised in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications.Cytochrome P450 1A1 is known for its role in the activation and detoxification of environmental procarcinogens, making it a target for investigations in cancer research and toxicology. By binding to the cytochrome P450 1A1 protein, this antibody enables precise detection and analysis in a variety of cell types, facilitating studies on drug metabolism, environmental toxicity, and the development of novel therapeutic interventions.
Understanding the activity and expression of cytochrome P450 1A1 is essential for elucidating its involvement in drug metabolism, carcinogenesis, and toxicology. The use of the Cytochrome P450 1A1 Polyclonal Antibody (CAB2159) provides researchers with a reliable tool to explore the function and regulation of this important enzyme, ultimately contributing to advancements in personalized medicine and the treatment of various diseases.
Product Name:
CYP1A1 Rabbit Polyclonal Antibody
SKU:
CAB2159
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-460 of human CYP1A1 (NP_000490.1).
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
1543
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using CYP1A1 Rabbit pAb (CAB2159) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
