The CYP17A1 Polyclonal Antibody (CAB13968) is a valuable tool for researchers studying the cytochrome P450 enzyme CYP17A1, which plays a crucial role in steroid hormone biosynthesis. Raised in rabbits, this antibody is highly reactive with human samples and is validated for use in Western blot applications. It specifically binds to the CYP17A1 protein, enabling accurate detection and analysis in a variety of cell types.CYP17A1 is involved in the biosynthesis of steroid hormones, including glucocorticoids, mineralocorticoids, and sex hormones such as testosterone. Dysregulation of CYP17A1 activity has been linked to various diseases, including hormone-dependent cancers like prostate and breast cancer.
Understanding the role of CYP17A1 in steroid hormone biosynthesis is essential for developing targeted therapies for these conditions.Overall, the CYP17A1 Polyclonal Antibody provides researchers with a reliable tool to investigate the function and regulation of the CYP17A1 enzyme in both health and disease. Its high specificity and reactivity make it a valuable asset for studies in endocrinology, oncology, and reproductive biology.
Product Name:
CYP17A1 Rabbit Polyclonal Antibody
SKU:
CAB13968
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 209-508 of human CYP17A1 (NP_000093.1).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.
Purification Method:
Affinity purification
Gene ID:
1586
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of rat testis, using CYP17A1 antibody (CAB13968) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
