The CYP11B1 Polyclonal Antibody (CAB7664) is a valuable tool for researchers studying the cytochrome P450 enzyme CYP11B1, which plays a crucial role in the biosynthesis of steroid hormones. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CYP11B1 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in endocrinology and hormone-related research.CYP11B1 is essential for the conversion of cholesterol to cortisol and aldosterone, two key hormones involved in the body's stress response and electrolyte balance.
Dysregulation of CYP11B1 activity has been linked to various endocrine disorders, including adrenal insufficiency and hypertension. Therefore, studying the expression and function of CYP11B1 is crucial for advancing our understanding of these conditions and exploring potential therapeutic strategies. The CYP11B1 Polyclonal Antibody offers reliable detection and characterization of CYP11B1, facilitating valuable insights into hormone biosynthesis and related pathologies.
Product Name:
CYP11B1 Rabbit Polyclonal Antibody
SKU:
CAB7664
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B1 (NP_000488.3).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
1584
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CYP11B1 antibody (CAB7664) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
