The CYP11A1 Polyclonal Antibody is a crucial tool for researchers studying the CYP11A1 protein, an enzyme involved in the biosynthesis of steroid hormones such as cortisol and aldosterone. This antibody is raised in rabbits and is highly specific to human samples, making it ideal for use in Western blot applications. CYP11A1 is essential for the production of steroid hormones in the adrenal glands and gonads, making it a key player in endocrine regulation and steroidogenesis. Dysregulation of CYP11A1 has been linked to various hormone-related disorders, including adrenal insufficiency and hormone-dependent cancers.
By using the CYP11A1 Polyclonal Antibody, researchers can accurately detect and analyze the expression of CYP11A1 in different cell types and tissues, providing valuable insights into its role in hormone synthesis and regulation. This antibody is a valuable tool for studies in endocrinology, reproductive biology, and hormone-related diseases, offering the potential for new therapeutic approaches and diagnostic strategies in the field of hormone research.
Product Name:
CYP11A1 Polyclonal Antibody
SKU:
CAB21757
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CYP11A1 (NP_000772.2).
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.
Purification Method:
Affinity purification
Gene ID:
1583
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CYP11A1 antibody (CAB21757) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
