The Cyclin T1 Polyclonal Antibody (CAB2057) is a valuable tool for researchers studying Cyclin T1, a crucial protein involved in regulating transcription in various cellular processes. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Cyclin T1 protein, this antibody enables researchers to detect and analyze its expression in different cell types, making it an ideal choice for studies in molecular biology and cancer research.
Cyclin T1 plays a key role in regulating the transcriptional elongation of RNA polymerase II, making it essential for the expression of genes involved in cell growth and development. Dysregulation of Cyclin T1 has been associated with various diseases, including cancer and viral infections, highlighting the importance of studying its function. By understanding the role of Cyclin T1, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.
Product Name:
Cyclin T1 Rabbit Polyclonal Antibody
SKU:
CAB2057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 220-320 of human Cyclin T1 (NP_001231.2).
Sequence:
HWWE YVDA TVTL ELLD ELTH EFLQ ILEK TPNR LKRI WNWR ACEA AKKT KADD RGTD EKTS EQTI LNMI SQSS SDTT IAGL MSMS TSTT SAVP SLPV SEES S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
CCNT; CYCT1; HIVE1; Cyclin T1
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
81kDa
Observed MW:
81kDa
This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
904
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Cyclin T1 Rabbit pAb (CAB2057) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
