The Cyclin H Monoclonal Antibody is a crucial tool for researchers studying cell cycle regulation and cancer development. This antibody, developed by Assay Genie, specifically targets cyclin H, a key protein involved in cell cycle progression and DNA repair processes.Cyclin H is known to form complexes with CDK7 and MAT1, contributing to the activation of cyclin-dependent kinases (CDKs) and ultimately regulating cell cycle checkpoints. Dysregulation of cyclin H has been linked to various cancer types, making it a promising target for therapeutic intervention.This monoclonal antibody is highly specific and sensitive, allowing for accurate detection and analysis of cyclin H levels in various cell types and tissues.
It is validated for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, providing researchers with valuable insights into the role of cyclin H in cellular processes.By using the Cyclin H Monoclonal Antibody, researchers can further explore the mechanisms underlying cell cycle control and cancer progression, ultimately contributing to the development of novel therapeutic strategies for cancer treatment. Don't miss out on the opportunity to advance your research with this high-quality antibody from Assay Genie.
Product Name:
Cyclin H Monoclonal Antibody
SKU:
CAB21885
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cyclin H (P51946).
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
902
Clone Number:
ARC2823
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cyclin H antibody (CAB21885) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
